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Sangon Biotech mutant probes
Mutant Probes, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutant+probes/mutant+probes/pm41596899-86-3-21
Average 86 stars, based on 1 article reviews
mutant probes - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Mutagenesis:

Article Title: EjMYB15 Improves Cold Tolerance of Postharvest Loquat Fruit via Upregulating Antioxidant Enzyme Genes
Article Snippet: .. Wild-type (WT) or mutant probes containing MYB binding sites of target gene promoters were synthesized and 3′-end labeled with biotin by Sangon Biotech (Shanghai, China). .. The EMSA was performed using the Light Shift Chemiluminescent EMSA Kit (Beyotime Biotechnology, Nanjing, China) and detected by a ChemiDocTM MP Imaging System (Bio-Rad, USA) using the chemiluminescence method [ ].

Article Title: Transcription factor PbMYB80 regulates lignification of stone cells and undergoes RING finger protein PbRHY1-mediated degradation in pear fruit.
Article Snippet: The Chinese white pear (Pyrus bretschneideri) fruit carries a high proportion of stone cells, adversely affecting fruit quality.. Lignin is a main component of stone cells in pear fruit.. In this study, we discovered that a pear MYB transcription factor, PbMYB80, binds to the promoters of key lignin biosynthesis genes and inhibits their expression.

Article Title: EjMYB15 Improves Cold Tolerance of Postharvest Loquat Fruit via Upregulating Antioxidant Enzyme Genes.
Article Snippet: .. Wild-type (WT) or mutant probes containing MYB binding sites of target gene promoters were synthesized and 3′-end labeled with biotin by Sangon Biotech https://doi.org/10.3390/foods15020301 (Shanghai, China). .. The EMSA was performed using the Light Shift Chemiluminescent EMSA Kit (Beyotime Biotechnology, Nanjing, China) and detected by a ChemiDocTM MP Imaging System (Bio-Rad, USA) using the chemiluminescence method [32].

Article Title: Self S-RNase suppresses PbrWHY2 and downstream target genes to inhibit pollen tube growth in pear.
Article Snippet: S-RNase-based gametophytic self-incompatibility (GSI) in flowering plants has been extensively studied, yet its underlying mechanisms remain incompletely understood.. Here, we show that overexpression of PbrWHY2, a WHIRLY transcription factor, promotes pear pollen tube growth, sustains reactive oxygen species (ROS) levels and enhances cellulose synthesis in the tube wall.. Conversely, silencing PbrWHY2 yields the opposite phenotype.

Article Title: The defensin gene NaDLP1, which confers resistance against Alternaria alternata, is transcriptionally regulated by NaWRKY3/6 in Nicotiana attenuata.
Article Snippet: .. Biotin-labeled DNA probes and mutant probes corresponding to 249 putative W-box elements in the NaDLP1 promoter were synthesized by 250 Sangon Biotech. ..

Article Title: The defensin gene NaDLP1 , which confers resistance against Alternaria alternata , is transcriptionally regulated by NaWRKY3/6 in Nicotiana attenuata
Article Snippet: .. Biotin-labeled DNA probes and mutant probes corresponding to putative W-box elements in the NaDLP1 promoter were synthesized by Sangon Biotech. ..

Binding Assay:

Article Title: EjMYB15 Improves Cold Tolerance of Postharvest Loquat Fruit via Upregulating Antioxidant Enzyme Genes
Article Snippet: .. Wild-type (WT) or mutant probes containing MYB binding sites of target gene promoters were synthesized and 3′-end labeled with biotin by Sangon Biotech (Shanghai, China). .. The EMSA was performed using the Light Shift Chemiluminescent EMSA Kit (Beyotime Biotechnology, Nanjing, China) and detected by a ChemiDocTM MP Imaging System (Bio-Rad, USA) using the chemiluminescence method [ ].

Article Title: EjMYB15 Improves Cold Tolerance of Postharvest Loquat Fruit via Upregulating Antioxidant Enzyme Genes.
Article Snippet: .. Wild-type (WT) or mutant probes containing MYB binding sites of target gene promoters were synthesized and 3′-end labeled with biotin by Sangon Biotech https://doi.org/10.3390/foods15020301 (Shanghai, China). .. The EMSA was performed using the Light Shift Chemiluminescent EMSA Kit (Beyotime Biotechnology, Nanjing, China) and detected by a ChemiDocTM MP Imaging System (Bio-Rad, USA) using the chemiluminescence method [32].

Synthesized:

Article Title: EjMYB15 Improves Cold Tolerance of Postharvest Loquat Fruit via Upregulating Antioxidant Enzyme Genes
Article Snippet: .. Wild-type (WT) or mutant probes containing MYB binding sites of target gene promoters were synthesized and 3′-end labeled with biotin by Sangon Biotech (Shanghai, China). .. The EMSA was performed using the Light Shift Chemiluminescent EMSA Kit (Beyotime Biotechnology, Nanjing, China) and detected by a ChemiDocTM MP Imaging System (Bio-Rad, USA) using the chemiluminescence method [ ].

Article Title: Transcription factor PbMYB80 regulates lignification of stone cells and undergoes RING finger protein PbRHY1-mediated degradation in pear fruit.
Article Snippet: The Chinese white pear (Pyrus bretschneideri) fruit carries a high proportion of stone cells, adversely affecting fruit quality.. Lignin is a main component of stone cells in pear fruit.. In this study, we discovered that a pear MYB transcription factor, PbMYB80, binds to the promoters of key lignin biosynthesis genes and inhibits their expression.

Article Title: EjMYB15 Improves Cold Tolerance of Postharvest Loquat Fruit via Upregulating Antioxidant Enzyme Genes.
Article Snippet: .. Wild-type (WT) or mutant probes containing MYB binding sites of target gene promoters were synthesized and 3′-end labeled with biotin by Sangon Biotech https://doi.org/10.3390/foods15020301 (Shanghai, China). .. The EMSA was performed using the Light Shift Chemiluminescent EMSA Kit (Beyotime Biotechnology, Nanjing, China) and detected by a ChemiDocTM MP Imaging System (Bio-Rad, USA) using the chemiluminescence method [32].

Article Title: Self S-RNase suppresses PbrWHY2 and downstream target genes to inhibit pollen tube growth in pear.
Article Snippet: S-RNase-based gametophytic self-incompatibility (GSI) in flowering plants has been extensively studied, yet its underlying mechanisms remain incompletely understood.. Here, we show that overexpression of PbrWHY2, a WHIRLY transcription factor, promotes pear pollen tube growth, sustains reactive oxygen species (ROS) levels and enhances cellulose synthesis in the tube wall.. Conversely, silencing PbrWHY2 yields the opposite phenotype.

Article Title: The defensin gene NaDLP1, which confers resistance against Alternaria alternata, is transcriptionally regulated by NaWRKY3/6 in Nicotiana attenuata.
Article Snippet: .. Biotin-labeled DNA probes and mutant probes corresponding to 249 putative W-box elements in the NaDLP1 promoter were synthesized by 250 Sangon Biotech. ..

Article Title: The defensin gene NaDLP1 , which confers resistance against Alternaria alternata , is transcriptionally regulated by NaWRKY3/6 in Nicotiana attenuata
Article Snippet: .. Biotin-labeled DNA probes and mutant probes corresponding to putative W-box elements in the NaDLP1 promoter were synthesized by Sangon Biotech. ..

Labeling:

Article Title: EjMYB15 Improves Cold Tolerance of Postharvest Loquat Fruit via Upregulating Antioxidant Enzyme Genes
Article Snippet: .. Wild-type (WT) or mutant probes containing MYB binding sites of target gene promoters were synthesized and 3′-end labeled with biotin by Sangon Biotech (Shanghai, China). .. The EMSA was performed using the Light Shift Chemiluminescent EMSA Kit (Beyotime Biotechnology, Nanjing, China) and detected by a ChemiDocTM MP Imaging System (Bio-Rad, USA) using the chemiluminescence method [ ].

Article Title: EjMYB15 Improves Cold Tolerance of Postharvest Loquat Fruit via Upregulating Antioxidant Enzyme Genes.
Article Snippet: .. Wild-type (WT) or mutant probes containing MYB binding sites of target gene promoters were synthesized and 3′-end labeled with biotin by Sangon Biotech https://doi.org/10.3390/foods15020301 (Shanghai, China). .. The EMSA was performed using the Light Shift Chemiluminescent EMSA Kit (Beyotime Biotechnology, Nanjing, China) and detected by a ChemiDocTM MP Imaging System (Bio-Rad, USA) using the chemiluminescence method [32].



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Image Search Results


miR-516b-5p is a miRNA binding to hsa_circ_0072107. (A) Venn diagram showed that two miRNAs may bind to hsa_circ_0072107. (B) The binding site information of miR-516b-5p and miR-510-5p on hsa_circ_0072107. (C) Reverse transcription-quantitative PCR validation of transfection efficiency in cells transfected with miR-NC, miR-516b-5p mimics and inhibitors. (D) hsa_circ_0072107, miR-516b-5p and miR-510-5p levels in RNA enriched by circ_0072107 probe or scramble probe during biotinylated probe pull-down assay. (E) hsa_circ_0072107 level in miRNA complexes enriched by anti-AGO or IgG during anti-AGO2 RIP assay. AC16 cells transfected with miR-516b-5p mimic or miR-NC were used. (F) The effect of miR-516b-5p mimic or miR-NC on the relative luciferase activity of luciferase reporter with linear circ_0072107 or linear mutcirc_0072107 as the artificial 3′ UTR of Renilla , respectively. *P<0.05, **P<0.01, n=3. miRNA, microRNA; AGO2, argonaute 2; RIP, RNA immunoprecipitation; NC, negative control.

Journal: Molecular Medicine Reports

Article Title: circRNA hsa_circ_0072107 aggravates myocardial hypertrophy via its function as a competitive endogenous RNA of miR-516b-5p

doi: 10.3892/mmr.2025.13597

Figure Lengend Snippet: miR-516b-5p is a miRNA binding to hsa_circ_0072107. (A) Venn diagram showed that two miRNAs may bind to hsa_circ_0072107. (B) The binding site information of miR-516b-5p and miR-510-5p on hsa_circ_0072107. (C) Reverse transcription-quantitative PCR validation of transfection efficiency in cells transfected with miR-NC, miR-516b-5p mimics and inhibitors. (D) hsa_circ_0072107, miR-516b-5p and miR-510-5p levels in RNA enriched by circ_0072107 probe or scramble probe during biotinylated probe pull-down assay. (E) hsa_circ_0072107 level in miRNA complexes enriched by anti-AGO or IgG during anti-AGO2 RIP assay. AC16 cells transfected with miR-516b-5p mimic or miR-NC were used. (F) The effect of miR-516b-5p mimic or miR-NC on the relative luciferase activity of luciferase reporter with linear circ_0072107 or linear mutcirc_0072107 as the artificial 3′ UTR of Renilla , respectively. *P<0.05, **P<0.01, n=3. miRNA, microRNA; AGO2, argonaute 2; RIP, RNA immunoprecipitation; NC, negative control.

Article Snippet: Biotin-labelled miR-516b-5p probe (5′-bio-AUCUGGAGGUAAGAAGCACUUU-3′) or biotin-labelled mutant miR-516b-5p probe (5′-bio-ACUCAAGAGUAAGAAGCACUUU-3′; Suzhou GenePharma Co., Ltd.) were incubated with cell lysates, and the pull-down assay was carried out using the Pierce Magnetic RNA-Protein Pull-Down Kit (Pierce; Thermo Fisher Scientific, Inc.).

Techniques: Binding Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Biomarker Discovery, Transfection, Pull Down Assay, Luciferase, Activity Assay, RNA Immunoprecipitation, Negative Control

Overexpression of hsa_circ_0072107 with mutant miR-516b-5p binding site cannot induce AC16 hypertrophy. (A) miR-516b-5p expression level in AC16 cells transfected with empty vector (vector), hsa_circ_0072107 overexpression vector (ov-circ_0072107) and overexpression vector of hsa_circ_0072107 with mutant miR-516b-5p binding site (mutov-circ_0072107). No significant difference was observed in (B) cell size (scale bars, 50 µm), (C) protein/DNA ratio, or (D) levels of BNP and β-MHC between the vector and mutov-circ_0072107 groups. miRNA, microRNA.

Journal: Molecular Medicine Reports

Article Title: circRNA hsa_circ_0072107 aggravates myocardial hypertrophy via its function as a competitive endogenous RNA of miR-516b-5p

doi: 10.3892/mmr.2025.13597

Figure Lengend Snippet: Overexpression of hsa_circ_0072107 with mutant miR-516b-5p binding site cannot induce AC16 hypertrophy. (A) miR-516b-5p expression level in AC16 cells transfected with empty vector (vector), hsa_circ_0072107 overexpression vector (ov-circ_0072107) and overexpression vector of hsa_circ_0072107 with mutant miR-516b-5p binding site (mutov-circ_0072107). No significant difference was observed in (B) cell size (scale bars, 50 µm), (C) protein/DNA ratio, or (D) levels of BNP and β-MHC between the vector and mutov-circ_0072107 groups. miRNA, microRNA.

Article Snippet: Biotin-labelled miR-516b-5p probe (5′-bio-AUCUGGAGGUAAGAAGCACUUU-3′) or biotin-labelled mutant miR-516b-5p probe (5′-bio-ACUCAAGAGUAAGAAGCACUUU-3′; Suzhou GenePharma Co., Ltd.) were incubated with cell lysates, and the pull-down assay was carried out using the Pierce Magnetic RNA-Protein Pull-Down Kit (Pierce; Thermo Fisher Scientific, Inc.).

Techniques: Over Expression, Mutagenesis, Binding Assay, Expressing, Transfection, Plasmid Preparation

ZFP36 is a target gene of miR-516b-5p. (A) Venn diagram identified differentially expressed target genes of miR-516b-5p. In the GSE32453 dataset, 461 differentially expressed mRNAs were identified in the myocardium of patients with hypertrophic cardiomyopathy. (B) The binding sites of miR-516b-5p on ZFP36 3′UTR. (C) ZFP36 mRNA level in RNA enriched by miR-516b-5p probe or mutant miR-516b-5p probe in a biotinylated probe pull-down assay. (D) The effect of miR-NC or miR-516b-5p mimic on the relative luciferase activity of luciferase reporter using ZFP36 3′UTR or mutant ZFP36 3′UTR (mutZFP36 3′UTR) as the artificial 3′ UTR of Renilla . (E) The protein level of ZFP36 in AC16 cells transfected with miR-NC, miR-516b-5p, miR-NC inhibitor, or miR-516b-5p inhibitor. *P<0.05, n=3. ZFP36, zinc ring finger protein 36; miRNA, microRNA; NC, negative control.

Journal: Molecular Medicine Reports

Article Title: circRNA hsa_circ_0072107 aggravates myocardial hypertrophy via its function as a competitive endogenous RNA of miR-516b-5p

doi: 10.3892/mmr.2025.13597

Figure Lengend Snippet: ZFP36 is a target gene of miR-516b-5p. (A) Venn diagram identified differentially expressed target genes of miR-516b-5p. In the GSE32453 dataset, 461 differentially expressed mRNAs were identified in the myocardium of patients with hypertrophic cardiomyopathy. (B) The binding sites of miR-516b-5p on ZFP36 3′UTR. (C) ZFP36 mRNA level in RNA enriched by miR-516b-5p probe or mutant miR-516b-5p probe in a biotinylated probe pull-down assay. (D) The effect of miR-NC or miR-516b-5p mimic on the relative luciferase activity of luciferase reporter using ZFP36 3′UTR or mutant ZFP36 3′UTR (mutZFP36 3′UTR) as the artificial 3′ UTR of Renilla . (E) The protein level of ZFP36 in AC16 cells transfected with miR-NC, miR-516b-5p, miR-NC inhibitor, or miR-516b-5p inhibitor. *P<0.05, n=3. ZFP36, zinc ring finger protein 36; miRNA, microRNA; NC, negative control.

Article Snippet: Biotin-labelled miR-516b-5p probe (5′-bio-AUCUGGAGGUAAGAAGCACUUU-3′) or biotin-labelled mutant miR-516b-5p probe (5′-bio-ACUCAAGAGUAAGAAGCACUUU-3′; Suzhou GenePharma Co., Ltd.) were incubated with cell lysates, and the pull-down assay was carried out using the Pierce Magnetic RNA-Protein Pull-Down Kit (Pierce; Thermo Fisher Scientific, Inc.).

Techniques: Binding Assay, Mutagenesis, Pull Down Assay, Luciferase, Activity Assay, Transfection, Negative Control

Effect of miR-516b-5p overexpression on AC16 hypertrophy and ZFP36 expression was blocked by hsa_circ_0072107 overexpression. Under ISO treatment, hsa_circ_0072107 overexpression vector (ov-circ_0072107), miR-516b-5p inhibitor, miR-516b-5p mimic (miR-516b-5p), or miR-516b-5p mimic plus ov-circ_0072107 was transfected into AC16 cells, respectively and the transfection reagent was used as negative control (ISO + NC). (A) miR-516b-5p expression level effects on (B) cell size (scale bars, 50 µm), (C) protein/DNA ratio and (D) the protein levels of BNP and β-MHC were measured. (E and F) The protein level of ZFP36 in different groups was detected by western blotting. (G) Relative ZFP36 levels measured by reverse transcription-quantitative PCR in different experimental groups. (H) Western blot analysis of ZFP36 protein expression in different experimental groups, with quantification of protein expression levels shown in the histogram. *P<0.05, n=3. miRNA, microRNA; ZFP36, zinc ring finger protein 36; ISO, isoproterenol; NC, negative control; vector group, cells transfected with empty vector; ov-circ_0072107, cells transfected with hsa_circ_0072107 overexpression vector; si-NC group, cells transfected with negative siRNA; si-circ_0072107 group, cells transfected with siRNA targeting hsa_circ_0072107 (si-circ_0072107).

Journal: Molecular Medicine Reports

Article Title: circRNA hsa_circ_0072107 aggravates myocardial hypertrophy via its function as a competitive endogenous RNA of miR-516b-5p

doi: 10.3892/mmr.2025.13597

Figure Lengend Snippet: Effect of miR-516b-5p overexpression on AC16 hypertrophy and ZFP36 expression was blocked by hsa_circ_0072107 overexpression. Under ISO treatment, hsa_circ_0072107 overexpression vector (ov-circ_0072107), miR-516b-5p inhibitor, miR-516b-5p mimic (miR-516b-5p), or miR-516b-5p mimic plus ov-circ_0072107 was transfected into AC16 cells, respectively and the transfection reagent was used as negative control (ISO + NC). (A) miR-516b-5p expression level effects on (B) cell size (scale bars, 50 µm), (C) protein/DNA ratio and (D) the protein levels of BNP and β-MHC were measured. (E and F) The protein level of ZFP36 in different groups was detected by western blotting. (G) Relative ZFP36 levels measured by reverse transcription-quantitative PCR in different experimental groups. (H) Western blot analysis of ZFP36 protein expression in different experimental groups, with quantification of protein expression levels shown in the histogram. *P<0.05, n=3. miRNA, microRNA; ZFP36, zinc ring finger protein 36; ISO, isoproterenol; NC, negative control; vector group, cells transfected with empty vector; ov-circ_0072107, cells transfected with hsa_circ_0072107 overexpression vector; si-NC group, cells transfected with negative siRNA; si-circ_0072107 group, cells transfected with siRNA targeting hsa_circ_0072107 (si-circ_0072107).

Article Snippet: Biotin-labelled miR-516b-5p probe (5′-bio-AUCUGGAGGUAAGAAGCACUUU-3′) or biotin-labelled mutant miR-516b-5p probe (5′-bio-ACUCAAGAGUAAGAAGCACUUU-3′; Suzhou GenePharma Co., Ltd.) were incubated with cell lysates, and the pull-down assay was carried out using the Pierce Magnetic RNA-Protein Pull-Down Kit (Pierce; Thermo Fisher Scientific, Inc.).

Techniques: Over Expression, Expressing, Plasmid Preparation, Transfection, Negative Control, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction